8.3 Salvaging Moldy Material

Sometimes things go poorly and you end with moldy material and wonder what you can do with it.  The answer is it depends on what kind of mold and how badly it's infected.

Molds belong to families like mushrooms, and like mushrooms all molds aren't created equal.  One of the biggest differences is whether they live on live or dead material.  Both Powdery Mildew and Botrytris are molds that live on living tissue, and produce no known aflatoxins, while molds like Aspergillus and Penicillin are composting molds living on dead tissue and do produce aflatoxins.

For the purposes of this discussion, aflatoxins are evil spirits that induce acute hepatic necrosis, resulting later in cirrhosis or carcinoma of the liver. 

See: https://www.ncbi.nlm.nih.gov/books/NBK350562/     and

http://poisonousplants.ansci.cornell.edu/toxicagents/aflatoxin/aflatoxin.html

Even they don't produce aflatoxins, some folks can still have a Type 1 allergic reaction to Botrytis spores and mycelium and both Powdery Mildew and Botrytis have a strong moldy odor and taste that is off putting.

Fortunately that smell and taste is produced by the spores and mycelium, which can be filtered out using a 0.2 micron syringe filter.  Once gone, the smell and taste is gone as well, as is ostensibly any allergic reactions. 

We typically vacuum filter the alcohol solution using a #1 Whatman medium filter, followed by a 1 micron filter, and lastly a 0.2 micron syringe filter.

Here is an inexpensive vacuum filtration setup using a Buckner funnel, an Erlenmeyer vacuum flask, and an AC rotary vane vacuum pump.  Works well, but does require frequent oil changes in the vacuum pump.

 

 Buchner funnel vacuum filtration set up

 

 0.2 Micron syringe filter

An OK process for small amounts, but for industrial quantities, filter presses, followed by cross flow microfiltration can filter to 0.2 microns and can be sized accordingly. 

Not so easy with Aspergillus or Penicillin, which do produce aflatoxins and which can't be removed by filtration.  Folks can also have an allergic reaction to its spores

Aspergillus produces more serious health effects from allergic reactions to spores and mycelium,  as well as invasively colonating tissue like lungs, and from aflatoxins, but Penicillium sp. can cause keratitis as well as infections in the inner ear, respiratory system, and urinary tract. 
Allergic reactions to Aspergillus spores include both Type 1 allergic reactions, as well as Type III hypersensitivity, pneumonitis, and others.  A. fumigatus can induce both allergic

bronchopulmonary aspergillosis and allergic fungal sinusitis.

As opportunistic pathogens growing at body temperature, they are especially invasive to individuals with compromised immune systems.
Sooo, how do you tell the difference, beyond the clues of whether it started out moldy or molded during drying and curing?

There are a couple handy tools.  One is an NDT blacklight, and the other is a 1000X microscope.

 

NDT Blacklight

The NDT black light was developed for fluorescent penetrant and magnetic particle inspection, but works well for our application, which is to cause any aflatoxins or Aspergillus mold material to fluoresce green, giving an indication of their presence and severity.

The second tool is to visually identify the mold using a 1000X microscope.  Here is a picture of our trusty Zeiss, but a good modern alternative is one of the less expensive modern generation of 1000X USB microscopic cameras, but look for one with good optics at that magnification.

 

1000X Microscope

And here is what the three different molds look like at extreme magnification, as well as mounted and stained in the last, to better demonstrate the Aspergillum form to look for.

 

Powdery Mildew


 

Botrytis  Cinerea


 

Botrytis fruiting body

Aspergillus mold was named after the religious water sprinkler of the Holy Water called an aspergillum.  A look at the fruiting body suggests why.

 

Aspergillium Holy Water sprinkler

 

Aspergillus fruiting body resembling an Aspergillum


 

Aspergillus spore cluster


Although a bacteria vis a vis a mold, Clostridium have also been found in poorly cured cannabis.  The Clostridium family of pathogens cause food botulism, as well as food poisoning, gas gangrene, and tenanus.


Filtering to 0.2 microns will remove those bacteria, but not their toxins.


https://web.archive.org/web/20150315040645/http://en.wikipedia.org/wiki/Clostridium

 


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